Vol. 3 No. 2 June 1998

Volume 3 (1998) pp 103-109
Title DOES THE APOPTOTIC FRAGMENTATION OF DNA STARTS BY EXCISION OF CHROMOSOMAL DNA LOOP DOMAINS?
Authors E. Ioudinkova, A. Bystritsky and S. Razin
Abstract In this paper we are presenting a critical review of works demonstrating that at the early stages of apoptosis eukaryotic DNA is cleaved to large fragments the length of which is several tens of thousands of nucleotide pairs. Evidence is presented that this fragmentation proceeds in a specific fashion and that the character of the fragmentation reflects the general principle of eukaryotic genome organization into structural-functional domains.
Address and Contact Information Institute of Gene Biology, 34/5 Vavilov St., 117334, Moscow, Russia
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Volume 3 (1998) pp 111-117
Title SIMPLIFIED SOLUTION OF LACTATE DEHYDROGENASE CATALYSED REACTION
Authors M. Kuczek
Abstract The lactate dehydrogenase catalysed reaction shows lag phase. This lag phase is easy for explanation if the consecution of first and second order equilibrium reactions were assumed for calculation of pyruvate trace concentration. The same explanation was accepted for calculation of significant pyruvate concentration. For calculation significant pyruvate concentration the consecution of simple Michaelis-Menten type and second order reactions was assumed. The exact solution of reaction rate equations system for this complex reaction was counted.
Address and Contact Information Institute of Chemistry, University of Opole
Oleska 48, 45-052 Opole, Poland
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Volume 3 (1998) pp 119-132
Title SYNTHESIS OF AMINOPHOSPHOLIPIDS IN Saccharomyces cerevisiae AND CHINESE HAMSTER OVARY CELLS. FROM MUTAGENESIS TO GENES AND CELLULAR FUNCTION
Authors J. Lenart and S. Pikula*
Abstract The purpose of this article is to provide a concise overview of the characterization of auxotrophic mutated cells to the precursors of lipid synthesis, and of the identification of specific genes encoding enzymatic proteins involved in this process. The focus is on enzymes catalyzing the synthesis of phosphatidylserine and phosphatidylethanolamine in Saccharomyces cerevisiae and Chinese hamster ovary cells, two cell types frequently used by investigators studying the mechanisms of genetic control of metabolic processes.
Address and Contact Information Laboratory of Lipid Biochemistry, Department of Cellular Biochemistry,
Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland
* Corresponding author. Fax: (+4822) 822 5342; E-mail: slawek@nencki.gov.pl
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Volume 3 (1998) pp 133-143
Title THE EFFECT OF POLYETHYLENE GLYCOL ON SOME PROPERTIES OF ERYTHROCYTE MEMBRANE
Authors M. Danieluk 1,2 H. Hagerstrand3, A. Jezierski 4, J. Jezierska4 and A. F. Sikorski1
Abstract Polyethylene glycol (PEG) induces fusion of cells creating fused cell pairs and larger cell aggregates. However the precise mechanism of its action on cell membranes remains unclear. In the present study we attempted to determine how PEG interacts with the membrane of red blood cell. It is known that PEG, at concentrations that evoke cell fusion, induces strong swelling of erythrocytes which in appeared to be insensitive to elevated ionic strength of the solution. This swelling, as well as fusion rate, is independent of the initial shape of erythrocytes induced by various amphiphiles. PEG in the concentrations used usually as a fusogen evoked haemolysis (up to 50%). Again, this effect was not inhibited by elevated ionic strength of the buffer. Further experiments revealed that PEG changes the membrane properties such as surface pressure of lipid monolayer prepared from total erythrocyte lipids and mobility of acyl hydrocarbon chains of membrane lipids as measured using 5-doxyl stearate as a spin probe.
Address and Contact Information 1University of Wroclaw, Institute of Biochemistry, Laboratory of Cell Biology, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland,
2Nencki Institute of Experimental Biology, Department of Cellular Biochemistry, Pasteura 3, 02093 Warsaw, Poland,
3Abo Akademi University, Department of Biology, Biocity 20520 Abo/Turku, Finland,
4University of Wrocław, Institute of Chemistry, Joliot-Curie 14, 50383 Wrocław, Poland
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Volume 3 (1998) pp 145-150
Title TOROCYTES - A NEW CLASS OF VESICLE SHAPES
Authors H. Hagerstrand1, V. Kralj-Iglic2, M. Bobrowska-Hagerstrand2, K. Bialkowska3, B. Isomaa1 and A. Iglic4
Abstract Torocyte shaped endovesicles with a low relative volume were induced in human erythrocytes by polyethyleneglycol dodecylether (C12E8). It is suggested that the torocyte endovesicles are formed in a process where an initially stomatocytic invagination loses volume while maintaining its large surface area. The boundaries of the phase diagram of the observed torocytes are described.
Address and Contact Information 1Department of Biology, Abo Akademi University, FIN-20520, Abo/Turku, Finland,
2Institute of Biophsics, Medical Faculty, University of Ljubljana, SI-1000, Ljubljana,Slovenia,
3Institute of Biochemistry, University of Wroclaw, PL-51148, Wroclaw, Poland
and 4Laboratory of Applied Physics, Faculty of Electrical Engineering, University of Ljubljana, SI-1000, Ljubljana, Slovenia
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ERRATUM to the article published in Cellular & Molecular Biology Letters volume 3, number 2, 1998, pp 145-150
Article TOROCYTES - A NEW CLASS OF VESICLE SHAPES
Authors H. Hagerstrand1, V. Kralj-Iglic2, M. Bobrowska-Hagerstrand2, K. Bialkowska3, B. Isomaa1 and A. Iglic4
Abstract The formula (3) on page 147 should read:

C2 = ± y"(1 + y'2)-3/2

Volume 3 (1998) pp 151-221
Seminar Title W. MEJBAUM-KATZENELLENBOGEN'S SEMINARS ON MOLECULAR BIOLOGY. 5. MEMBRANE SKELETON. REGULATORY FUNCTION IN NORMAL AND ABNORMAL CELLS
Abstracts List CALCIUM IN SPECTRIN FUNCTION
L. Backman, S. Lundberg, A. Buevich , I. Sethson and U. Edlund - p.152

BETAII SPECTRINS AND FODAXINS: INTERACTING PROTEINS IN MANY TISSUES
A. J. Baines, V. Ohanian, E. Heerkens, G. Phillips and N. V. L. Hayes - p.153

REGULATION OF ANNEXIN VI FUNCTION BY ATP
J. Bandorowicz-Pikula, M. Danieluk, A. Wrzosek and S. Pikula - p.155

A SPECTRIN MEMBRANE SKELETON OF THE GOLGI COMPLEX
K. A. Beck - p.157

MOLECULAR PHYSIOLOGY OF THE ANKYRIN FAMILY: LESSONS FROM DISRUPTION OF ANKYRIN B AND ANKYRING GENES IN MICE
V. Bennett, D. Zhou, S. Tuvia, S. Lambert and - p.158

IDENTIFICATION OF A NOVEL, PROLINE-RICH PROTEIN WHICH INTERACTS WITH a-SPECTRIN SH3 DOMAIN
K. Bialkowska, V-P. Lehto and J.E.B Fox - p.159

MOLECULAR BASIS OF RESISTANCE OF WINTER TRITICALE TO APHIDS
A. Piotr Ciepiela and M. Lubkowski - p.161

HEREDITARY DEHYDRATED STOMATOCYTOSIS : RECENT ADVANCES AND QUESTIONS
J. Delaunay, S. Grootenboer, O. Schischmanoff, T. Cynober, G. Tchernia, J.P.Dommergues, M. Bost, G. Stewart, S. Perrotta, M. Carella, - p.161

CRYSTAL STRUCTURE F-ACTIN BINDING DOMAIN FROM HUMAN b-SPECTRIN
K. Djinovic Carugo, S. Banuelos and M. Saraste - p.163

HEREDITARY ELLIPTOCYTOSIS: INFLUENCE OF TRANS-ACTING LOW EXPRESSION ALLELES ON DISEASE SEVERITY
S.W. Eber, D.C. Boemmer and S. Ullmann - p.164

REGULATION OF EXOCYTOSIS BY CYTOSKELETON IN NEUROENDOCRINE CELLS
S. Gasman, S. Chasserot-Golaz, M.F. Bader and D. Aunis - p.166

ANNEXINS AND MEMBRANE DYNAMICS
V. Gerke - p.167

THE SPECTRIN MEMBRANE SKELETON: A KEY ELEMENT IN SICKLE CELL DISEASE
S. R. Goodman - p.168

STRUCTURAL STUDIES OF CLONED SEGMENTS OF CHICKEN BRAIN a-SPECTRIN
V.L. Grum, D. Li, R.I. MacDonald, A. Mondragon and D.P. Pantazatos - p.169

THE LAMPREY (Lampetra fluviatilis) ERYTHROCYTE; MORPHOLOGY, ULTRASTRUCTURE, MAJOR PLASMA MEMBRANE PROTEINS AND PHOSPHOLIPIDS, AND CYTOSKELETAL ORGANIZATION
H. Hagerstrand, M. Danieluk and M. Nikinmaa - p.170

SEQUENCES HOMOLOGOUS TO ALPHA-SPECTRIN ARE EXPRESSED IN PLANTS
B. Hanus-Lorenz, A. Hryniewicz, M. Lorenz - p.171

THE STRUCTURE OF THE RESTING AND ACTIVATED PLATELET CYTOSKELETON
J. H. Hartwig, K. Barkalow, A. Azim, G. Bokoch, Y. Ohta, S. Nakamura, T. Stossel, K. Tolias and C. Carpenter - p.172

THE L1 FAMILY OF NEURAL CELL ADHESION MOLECULES - LINKING CELL ADHESION TO MEMBRANE SKELETON ORGANIZATION
M. Hortsch and R.R. Dubreuil - p.174

SPICULATED RED BLOOD CELLS INDUCED BY INTERCALATION OF AMPHIPHILES
A. Iglic , V. Kralj-Iglic and H. Hagerstrand - p.176

RATE CONSTANTS FOR REVERSIBLE DIDS BINDING TO BAND 3 PROTEIN IN HUMAN ERYTHROCYTE MEMBRANES
T. Janas and T. Janas - p.177

DECREASE OF PKCa TRANSLOCATION TO THE ERYTHROCYTE MEMBRANE IN PATIENTS WITH CHRONIC RENAL FAILURE (CRF)
J. Kaczmarek, G. Komorowska and M. Rybczynska - p.178

THE LYTIC ACTION OF POLYENE ANTIBIOTIC AMPHOTERICIN B ON HUMAN ERYTHROCYTES
A. Knopik-Skrocka and J. Bielawski - p.179

ACTIN-BINDING MEMBRANE PROTEINS IN MOTILE CELLS
E. J. Luna, R. K. Pope, K. N. Pestonjamasp and J. D.Wulfkuhle - p.180

EXPRESSION AND FUNCTION OF RED CELL PROTEIN 4.1 AND ITS HOMOLOGUES IN ERYTHROID AND NON-ERYTHROID CELLS
N. Mohandas, M. Parra, - p.181

SPECTROSCOPIC PROPERTIES OF SELECTED FLUORESCENCE PROBES USED FOR LABELLING OF LYMPHOCYTE OR BLAST CELL MEMBRANES AND PLASMA HIGH DENSITY LIPOPROTEINS
K. Michalak, A.Sidorowicz, M. Kuliszkiewicz-Janus, T. Modrzycka and A. Pola - p.182

PARTICIPATION OF INTEGRIN RECEPTORS IN SIGNAL TRANSDUCTION OF CONTACT INHIBITED C3H10T1/2 CELLS
J. Miloszewska and - p.184

APC GENE IS MUTATED IN ENDOMETRIAL CANCER OF WOMEN
R. Miturski, D. Burnouf, M. Nothisen, J. Tomaszewski, J. Jakowicki and R. Fuchs - p.185

THE EFFECT OF SODIUM TAURODEOXYCHOLATE ON HUMAN ERYTHROCYTES
L. Mrowczynska and J. Bielawski - p.186

INFLUENCE OF SOME POLYOLS ON ERYTHROCYTE CYTOSKELETON PROTEINS
O.A. Nardid, T.S. Dyubko, A.S. Soloviova and A.V. Zinchenko - p.187

TISSUE-DISTRIBUTION OF TWO a FODRIN ISOFORMS : CHARACTERIZATION AND STUDY OF THEIR SENSITIVITY TO CALPAIN
G. Nicolas, L. Malbert-Colas, H. Gautero, C. Fournier and M.C. Lecomte - p.189

STUDY OF DYNAMICS BEE VENOM - INDUCED SHRINKAGE OF ERYTHROCYTES OF THE PATIENTS WITH ANEMIAS OF VARIOUS ETIOLOGY
E.E. Nipot , S.B. Dyryavy and V.V. Cherepanov - p.191

MODULATED HUMAN GLOBIN GENE EXPRESSION: ROLE FOR ANTISENSE EXPRESSION VECTORS
B. S. Pace - p.192

MECHANICAL STRESS AND HEMIN PRETREATMENT EFFECTS ON KINETIC PARAMETERS OF ERYTHROCYTES RESPONSE TO HYPEROSMOTIC SHOCK
E.R. Pantaler and N.M. Shpakova - p.193

DETERMINATION OF THE CONTRACTILE MEMBRANE COMPLEX RESPONSIBLE FOR BEE VENOM-INDUCED SHRINKAGE OF THE ERYTHROCYTE MEMBRANE
T.A. Parchenko - p.194

ACTOMYOSIN MOTOR IN THE MEROZOITE OF THE MALARIA PARASITE, Plasmodium falciparum: IMPLICATIONS FOR RED CELL INVASION
J. C. Pinder - p.195

ERYTHROCYTE MEMBRANE SKELETON: A PUTATIVE PARTICIPATOR OF ADAPTIVE CELLULAR RESPONSE TO TEMPERATURE VARIATION
S.V. Repina and N.V. Repin - p.196

TECHNIQUE TO MONITOR MULTIPLE SHAPE TRANSFORMATION EVENTS CAUSED BY THE INTERACTION OF RED BLOOD CELLS WITH BIOLOGICALLY ACTIVE SUBSTANCES
S.V. Rudenko - p.198

THE ARRANGEMENT OF CORTICAL MICROTUBULES IN PLANT EPIDERMIS IS SENSITIVE TO THE CHANGES IN CELL WALL STRESSES
T. Rusin , A. Szeja and Z. Hejnowicz - p.200

NO INCREASE OF PHOSPHATIDYLSERINE EXPOSURE ACCOMPANYING THE INCREASED MEMBRANE FLUIDITY IN ERYTHROCYTES OF ATAXIA TELANGIECTASIA GENE (ATM) CARRIERS
M. Rybczynska, J. Kaczmarek and A. L. Pawlak - p.201

EXPRESSION OF c-erbB-2 ONCOPROTEIN IN PROLIFERATIVE AND HYPERPLASTIC HUMAN ENDOMETRIUM
A. Semczuk, R. Miturski, J. Tomaszewski, K. Postawski, J. Kotarski and J. Jakowicki - p.202

NEWER FACETS OF SICKLE CELL ADHESIVENESS
S. B. Shohet, and B. J.-M. Thevenin - p.203

INTERACTIONS OF SPECTRINS WITH PHOSPHOLIPIDS: POSSIBLE PHYSIOLOGICAL ROLE
A. F. Sikorski - p.205

STOMATOCYTOSIS AND STOMATIN
G. W. Stewart and S.E. Coles - p.206

CYTOSKELETON - BILAYER INTERACTION AND RED BLOOD CELL SHAPES
S. Svetina - p.208

CONGENITAL DYSERYTHROPOIETIC ANAEMIA TYPE III
A. Wahlin and H. Sandstrom - p.209

CASPASE AND CALPAIN MEDIATED PROTEOLYSIS OF NONERYTHROID SPECTRIN SUBUNITS IN NEURONAL CELLS UNDERGOING APOPTOSIS
K. K.W. Wang, N. Rathna, - p.210

NEW INFORMATION ON CONGENITAL DYSERYTHROPOIETIC ANAEMIA, TYPE I
S. N. Wickramasinghe - p.211

REGULATION OF SIGNAL TRANSDUCTION ACROSS SKELETON-ASSOCIATED ADHESION RECEPTORS IN PLATELETS
J. E.B. Fox and S. Kulkarni
not published in issue

FAP52 AND EAST , TWO NEW FOCAL ADHESION PROTEINS
O. Lohi, J. Meriläinen and V.-P. Lehto
not published in issue

  Full text of the abstracts
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